验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19815-1-AP targets SPG21 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag13851 Product name: Recombinant human SPG21 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-308 aa of BC000244 Sequence: MGEIKVSPDYNWFRGTVPLKKIIVDDDDSKIWSLYDAGPRSIRCPLIFLPPVSGTADVFFRQILALTGWGYRVIALQYPVYWDHLEFCDGFRKLLDHLQLDKVHLFGASLGGFLAQKFAEYTHKSPRVHSLILCNSFSDTSIFNQTWTANSFWLMPAFMLKKIVLGNFSSGPVDPMMADAIDFMVDRLESLGQSELASRLTLNCQNSYVEPHKIRDIPVTIMDVFDQSALSTEAKEEMYKLYPNARRAHLKTGGNFPYLCRSAEVNLYVQIHLLQFHGTKYAAIDPSMVSAEELEVQKGSLGISQEEQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spastic paraplegia 21 (autosomal recessive, Mast syndrome) |
| 别名 | ABHD21, Acid cluster protein 33, ACP33, BM 019, GL010 |
| 计算分子量 | 308 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC000244 |
| 基因名称 | SPG21 |
| Gene ID (NCBI) | 51324 |
| RRID | AB_3741970 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPG21, also known as Maspardin, is a protein encoded by the SPG21 gene on human chromosome 15q22.31. SPG21 is critical for the proper movement and positioning of lysosomes within neurons, which is essential for maintaining axonal health. Mutations in the SPG21 gene underlie hereditary spastic paraplegia (SPG) type 21 (also known as Mast syndrome). This is a complex form of SPG characterized by motor coordination defects due to axonal degeneration of corticospinal neurons and associated with dementia, cerebellar, and extrapyramidal abnormalities and, in some cases, seizures.(PMID: 41400694,PMID: 34492745)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPG21 antibody 19815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
