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验证数据展示

  • WB analysis of mouse testis using 17826-1-AP (same clone as 17826-1-PBS)

    WB analysis of mouse testis using 17826-1-AP (same clone as 17826-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    WB analysis of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    WB result of SPERT antibody (17826-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPERT transfected PC-3 cells. This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    IF Staining of PC-3 using 17826-1-AP (same clone as 17826-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17826-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPERT Polyclonal antibody, PBS Only

SPERT Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 17826-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17826-1-PBS targets SPERT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12200

Product name: Recombinant human SPERT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-412 aa of BC027609

Sequence: MQPEGLKCRMEESNAERGTAEPFPRLHNLYSTPRCAQQAALPRLSRRMASQHSYPLNRFSSVPLDPMERPMSQADLELDYNPPRVQLSDEMFVFQDGRWVNENCRLQSPYFSPSASFHHKLHHKRLAKECMLQEENKSLREENKALREENRMLSKENKILQVFWEEHKASLGREESRAPSPLLHKDSASLEVVKKDHVALQVPRGKEDSTLQLLREENRALQQLLEQKQAYWAQAEDTAAPAEESKPAPSPHEEPCSPGLLQDQGSGLSSRFEEPKGPPARQEDSKELRALRKMVSNMSGPSGEEEAKVGPGLPDGCQPLQLLREMRQALQALLKENRLLQEENRTLQVLRAEHRGFQEENKALWENNKLKLQQKLVIDTVTEVTARMEMLIEELYAFMPARSQDPKKPSRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatid associated
别名
计算分子量 448 aa, 47 kDa, 52 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC027609
基因名称 SPERT
Gene ID (NCBI) 220082
RRIDAB_2195674
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NA61
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPERT (spermatid associated protein), also known as CBY2 (chibby homolog 2) or NURIT, is a novel leucine-zipper protein belonging to the chibby family of proteins [PMID:12204287]. Uniquely expressed in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. It contains a leucine-zipper motif and two coiled-coil regions, and is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies. [PMID:12204287]

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