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验证数据展示

  • IHC staining of mouse heart using 18716-1-AP

    IHC staining of mouse heart using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 18716-1-AP

    IHC staining of mouse heart using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 18716-1-AP

    IHC staining of mouse skeletal muscle using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 18716-1-AP

    IHC staining of mouse skeletal muscle using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPEG Polyclonal antibody

SPEG Polyclonal Antibody for IHC, ELISA
Cat No. 18716-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

APEG 1, APEG1, BPEG, KIAA1297, SPEG, SPEG complex locus, SPEGalpha, SPEGbeta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18716-1-AP targets SPEG in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SPEG complex locus
别名 APEG 1, APEG1, BPEG, KIAA1297, SPEG, SPEG complex locus, SPEGalpha, SPEGbeta
计算分子量 354 kDa
GenBank蛋白编号NM_005876
基因名称 SPEG
Gene ID (NCBI) 10290
RRIDAB_3085566
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15772
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.

实验方案

Product Specific Protocols
IHC protocol for SPEG antibody 18716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols