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验证数据展示

  • WB analysis using 27989-1-AP (same clone as 27989-1-PBS)

    WB analysis using 27989-1-AP (same clone as 27989-1-PBS)

    Rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 27989-1-AP (SPEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

  • IP experiment of rat testis using 27989-1-AP (same clone as 27989-1-PBS)

    IP experiment of rat testis using 27989-1-AP (same clone as 27989-1-PBS)

    IP result of anti-SPEF2 (IP:27989-1-AP, 4ug; Detection:27989-1-AP 1:1000) with rat testis tissue lysate 1800 ug. This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 27989-1-AP (same clone as 27989-1-PBS)

    IF Staining of mouse testis using 27989-1-AP (same clone as 27989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SPEF2 antibody (27989-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27989-1-PBS in a different storage buffer formulation.

SPEF2 Polyclonal antibody, PBS Only

SPEF2 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA
Cat No. 27989-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, Indirect ELISA

别名

KPL2, Protein KPL2, sperm flagellar 2, Sperm flagellar protein 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27989-1-PBS targets SPEF2 in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27506

Product name: Recombinant human SPEF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 103-316 aa of NM_024867

Sequence: MALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm flagellar 2
别名 KPL2, Protein KPL2, sperm flagellar 2, Sperm flagellar protein 2
计算分子量 210 kDa
观测分子量 210 kDa
GenBank蛋白编号NM_024867
基因名称 SPEF2
Gene ID (NCBI) 79925
RRIDAB_3742269
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C093
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sperm flagellar protein 2 (SPEF2) is also named KPL2. As a cilia-associated protein, SPEF2 is broadly expressed in various cilia-related organs, such as the lung, spleen, trachea, brain, and testis (PMID: 10100999). SPEF2 is known to be essential for sperm tail development (PMID: 28619825). During spermatogenesis, SPEF2 is weakly expressed in cytoplasm in pachytene spermatocytes and round spermatocytes, then SPEF2 is localized in the manchette of steps 10-12 elongating spermatids, in the basal body at steps 13-14, and finally in the midpiece of the sperm tail at steps 14-16 (PMID: 19889948). Spef2 is expressed in the bone and cartilage, and Spef2 expression in osteoblasts increases during in vitro differentiation (PMID: 29339787).

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