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验证数据展示

  • WB analysis of Neuro-2a using 17836-1-AP

    WB analysis of Neuro-2a using 17836-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17836-1-AP (SPDYA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 17836-1-AP

    IP experiment of mouse testis using 17836-1-AP

    IP result of anti-SPDYA (IP:17836-1-AP, 3ug; Detection:17836-1-AP 1:300) with mouse testis tissue lysate 4000ug.

SPDYA Polyclonal antibody

SPDYA Polyclonal Antibody for WB, IP, ELISA
Cat No. 17836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17836-1-AP targets SPDYA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPDYA fusion protein Ag12041 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 speedy homolog A (Xenopus laevis)
别名 hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1
计算分子量 313 aa, 36 kDa
观测分子量 33 kDa
GenBank蛋白编号BC093005
基因名称 SPDYA
Gene ID (NCBI) 245711
RRIDAB_2878448
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5MJ70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for SPDYA antibody 17836-1-APDownload protocol
IP protocol for SPDYA antibody 17836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols