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SPDYA Polyclonal antibody

SPDYA Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17836-1-AP

Print datasheet

Synonyms

hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1

验证数据展示

  • WB analysis of Neuro-2a using 17836-1-AP

    WB analysis of Neuro-2a using 17836-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17836-1-AP (SPDYA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 17836-1-AP

    IP experiment of mouse testis using 17836-1-AP

    IP result of anti-SPDYA (IP:17836-1-AP, 3ug; Detection:17836-1-AP 1:300) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17836-1-AP is SPDYA Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen SPDYA fusion protein Ag12041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name speedy homolog A (Xenopus laevis)
Synonyms hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1
Calculated Molecular Weight 313 aa, 36 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC093005
Gene Symbol SPDYA
Gene ID (NCBI) 245711
RRIDAB_2878448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5MJ70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for SPDYA antibody 17836-1-APDownload protocol
IP protocol for SPDYA antibody 17836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols