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验证数据展示

  • WB analysis of Neuro-2a using 17836-1-AP

    WB analysis of Neuro-2a using 17836-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 17836-1-AP (SPDYA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 17836-1-AP

    IP experiment of mouse testis using 17836-1-AP

    IP result of anti-SPDYA (IP:17836-1-AP, 3ug; Detection:17836-1-AP 1:300) with mouse testis tissue lysate 4000ug.

SPDYA Polyclonal antibody

SPDYA Polyclonal Antibody for WB, IP, ELISA
Cat No. 17836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17836-1-AP targets SPDYA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12041

Product name: Recombinant human SPDYA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC093005

Sequence: MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 speedy homolog A (Xenopus laevis)
别名 hSpy/Ringo A, RINGO A, Ringo3, SPDY1, SPDYA, Speedy 1, Speedy protein A, SPY1
计算分子量 313 aa, 36 kDa
观测分子量 33 kDa
GenBank蛋白编号BC093005
基因名称 SPDYA
Gene ID (NCBI) 245711
RRIDAB_2878448
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5MJ70
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.

实验方案

Product Specific Protocols
WB protocol for SPDYA antibody 17836-1-APDownload protocol
IP protocol for SPDYA antibody 17836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols