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验证数据展示

  • WB analysis using 17640-1-AP

    WB analysis using 17640-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17640-1-AP (SPCS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 17640-1-AP

    IHC staining of rat liver using 17640-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 17640-1-AP

    IHC staining of human placenta using 17640-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17640-1-AP (SPCS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPCS3 Polyclonal antibody

SPCS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17640-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

PRO3567, SPase 22/23 kDa subunit, SPC22, SPC22/23, SPC3, SPCS3, YLR066W

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17640-1-AP targets SPCS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SPCS3 fusion protein Ag11780 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal peptidase complex subunit 3 homolog (S. cerevisiae)
别名 PRO3567, SPase 22/23 kDa subunit, SPC22, SPC22/23, SPC3, SPCS3, YLR066W
计算分子量 180 aa, 20 kDa
观测分子量23 kDa
GenBank蛋白编号BC047290
基因名称 SPCS3
Gene ID (NCBI) 60559
RRIDAB_3669279
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61009
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The microsomal signal peptidase complex (SPC), which is an essential membrane component in the endoplasmic reticulum (ER), has five subunits, including SEC11A, SEC11C, SPCS1, SPCS2, and SPCS3. Functionally, SPC removes signal peptides (SPs) from a series of secretory pre-proteins with high specificity. The dysregulation of the other SPC subunits was also observed in some tumors. SPCS3 expression can be used as a component of a risk score model to predict the survival of patients with pancreatic adenocarcinoma. (PMID: 35653344)

实验方案

Product Specific Protocols
WB protocol for SPCS3 antibody 17640-1-APDownload protocol
IHC protocol for SPCS3 antibody 17640-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols