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验证数据展示

  • WB analysis using 11847-1-AP

    WB analysis using 11847-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11847-1-AP (SPCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 11847-1-AP

    IF Staining of BxPC-3 using 11847-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using SPCS1 antibody (11847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

SPCS1 Polyclonal antibody

SPCS1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11847-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SPC12, SPC1, SPase 12 kDa subunit, HSPC033

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human skin tissue, PC-3 cells
Positive IF/ICC detected inBxPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11847-1-AP targets SPCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SPCS1 fusion protein Ag2407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal peptidase complex subunit 1 homolog (S. cerevisiae)
别名 SPC12, SPC1, SPase 12 kDa subunit, HSPC033
计算分子量 102 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC000884
基因名称 SPCS1
Gene ID (NCBI) 28972
RRIDAB_2195400
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6A9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPCS1 antibody 11847-1-APDownload protocol
IF protocol for SPCS1 antibody 11847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Authors - Rong Zhang
  • KO Validated
View Article
humanWB

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R
  • KD Validated
View Article
WB

PLoS Pathog

SPCS1-Dependent E2-p7 processing determines HCV Assembly efficiency.

Authors - Nabeel Alzahrani
View Article
humanWB

Mol Cell

Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway

Authors - Iqbal Dulloo
View Article