验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human skin tissue, PC-3 cells |
| Positive IF/ICC detected in | BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
产品信息
11847-1-AP targets SPCS1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2407 Product name: Recombinant human SPCS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-102 aa of BC000884 Sequence: MLEHLSSLPTQMDYKGQKLAEQMFQGIILFSAIVGFIYGYVAEQFGWTVYIVMAGFAFSCLAQLTLPPWPIYRRHPLKWLPVQESSTDDKKPGERKIKRHAK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal peptidase complex subunit 1 homolog (S. cerevisiae) |
| 别名 | SPC12, SPC1, SPase 12 kDa subunit, HSPC033 |
| 计算分子量 | 102 aa, 12 kDa |
| 观测分子量 | 12 kDa |
| GenBank蛋白编号 | BC000884 |
| 基因名称 | SPCS1 |
| Gene ID (NCBI) | 28972 |
| RRID | AB_2195400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6A9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SPCS1 antibody 11847-1-AP | Download protocol |
| WB protocol for SPCS1 antibody 11847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.
| ||
PLoS Pathog Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.
| ||
Mol Cell Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway |
