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验证数据展示

  • WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    WB analysis using 29930-1-AP (same clone as 29930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    IF Staining of HepG2 using 29930-1-AP (same clone as 29930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    IHC staining of human placenta using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    IHC staining of human lung cancer using 29930-1-AP (same clone as 29930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29930-1-PBS in a different storage buffer formulation.

SPATS2L Polyclonal antibody, PBS Only

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29930-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29930-1-PBS targets SPATS2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31157

Product name: Recombinant human SPATS2L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 290-488 aa of BC018736

Sequence: NYSSRTPCSSLLPLLNAHAATSGKQSNFSRKSSTHNKPSEGKAANPKMVSSLPSTADPSHQTMPANKQNGSSNQRRRFNPQYHNNRLNGPAKSQGSGNEAEPLGKGNSRHEHRRQPHNGFRPKNKGGAKNQEASLGMKTPEAPAHSEKPRRRQHAADTSEARPFRGSVGRVSQCNLCPTRIEVSTDAAVLSVPAVTLVA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 viral DNA polymerase-transactivated protein 6
别名
计算分子量 488 aa, 54 kDa
观测分子量54-65 kDa
GenBank蛋白编号BC018736
基因名称 SPATS2L
Gene ID (NCBI) 26010
RRIDAB_2923620
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUQ6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

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