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验证数据展示

  • WB analysis using 32517-1-AP

    WB analysis using 32517-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 32517-1-AP (SPATS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 32517-1-AP

    IP experiment of HeLa using 32517-1-AP

    IP result of anti-SPATS2 (IP:32517-1-AP, 4ug; Detection:32517-1-AP 1:5000) with HeLa cells lysate 1400 ug.

SPATS2 Polyclonal antibody

SPATS2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 32517-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

Nbla00526, p59scr, SCR59, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32517-1-AP targets SPATS2 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 SPATS2 fusion protein Ag38507 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated, serine-rich 2
别名 Nbla00526, p59scr, SCR59, Serine-rich spermatocytes and round spermatid 59 kDa protein, SPATA10
计算分子量60kDa，545aa
观测分子量70 kDa
GenBank蛋白编号NM_001293285.1
基因名称 SPATS2
Gene ID (NCBI) 65244
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86XZ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPATS2, also known as SCR59 and SPATA10, is a member of the RNA-binding proteins whose aberrant expression is associated with carcinogenesis in several types of cancer. It is mainly expressed in the adult testis, with low expression in liver and other tissues (PMID: 33037180). The apparent molecular weight is higher than the calculated molecular weight of 60 kDa, probably due to post-translational modification, probably by phosphorylation.

实验方案

Product Specific Protocols
WB protocol for SPATS2 antibody 32517-1-APDownload protocol
IP protocol for SPATS2 antibody 32517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols