验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse ovary tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SPATA6 Ready-To-Use IHC Kit. SPATA6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11849-1-AP targets SPATA6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2413 Product name: Recombinant human SPATA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-472 aa of BC020660 Sequence: YHLAPVEKSHGRLQNRTSRSQKKKSKSPERSKYCINAKNYEQPTISSKSHSPSPYTKRRMCELSEDTRRRLAHLNLGPYEFKKETDKPPFVIRHVDPPSPRADTLLGSSGRDCERDGWSRVHNDHSHLGCCRPKDYKVIRTPHGRDFDDSLEKCEEYLSPRSCSKPRHSARTLLVHSAPSTMPKHSPSPVLNRASLRERFHSDWCSPSNCDEIHDRDERDLEKDDELELKRSLLCRDSAYDSDPEYSSCQQPRGTFHLDDGEYWSNRAASYKGKSHRPIFENSMDKMYRNLYKKACSSASHTQESF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermatogenesis associated 6 |
| 别名 | HASH, SPATA6, spermatogenesis associated 6, SRF 1, SRF1 |
| 计算分子量 | 472 aa, 54 kDa |
| 观测分子量 | 54-56 kDa |
| GenBank蛋白编号 | BC020660 |
| 基因名称 | SPATA6 |
| Gene ID (NCBI) | 54558 |
| RRID | AB_11232594 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NWH7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| IP protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| WB protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Toxicol Appl Pharmacol Low dose lead exposure at the onset of puberty disrupts spermatogenesis-related gene expression and causes abnormal spermatogenesis in mouse. | ||
Theriogenology Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro. | ||
Clin Genet Identification of nonfunctional SPATA20 causing acephalic spermatozoa syndrome in humans | ||
Elife CCDC113 stabilizes sperm axoneme and head-tail coupling apparatus to ensure male fertility |
