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验证数据展示

  • WB analysis using 18414-1-AP

    WB analysis using 18414-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18414-1-AP (SPATA5L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18414-1-AP

    WB analysis of HeLa using 18414-1-AP

    WB result of SPATA5L1 antibody (18414-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPATA5L1 transfected HeLa cells.

SPATA5L1 Polyclonal antibody

SPATA5L1 Polyclonal Antibody for WB, ELISA
Cat No. 18414-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

SPATA5L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18414-1-AP targets SPATA5L1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SPATA5L1 fusion protein Ag13269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 5-like 1
别名 SPATA5L1
计算分子量 81 kDa
观测分子量81 kDa
GenBank蛋白编号BC000981
基因名称 SPATA5L1
Gene ID (NCBI) 79029
RRIDAB_2918062
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BVQ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPATA5L1 (Spermatogenesis-associated protein 5-like protein 1) belongs to the AAA+ATPase protein superfamily. A full-length SPATA5L1 transcript and a short SPATA5L1 variant can be produced in human by alternative splicing. Bi-allelic SPATA5L1 variants lead to a human disease characterized by sensorineural hearing loss with or without a nonprogressive mixed neurodevelopmental phenotype. And immunofluorescence analysis in rat hippocampal neurons demonstrated that SPATA5L1 was located in neuronal and glial cell nuclei, with more prominent expression in neurons (PubMed:34626583). SPATA5L1 also has 3 isoforms.

实验方案

Product Specific Protocols
WB protocol for SPATA5L1 antibody 18414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols