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验证数据展示

  • WB analysis using 32104-1-AP

    WB analysis using 32104-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32104-1-AP (SPATA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 32104-1-AP

    IHC staining of human liver using 32104-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 32104-1-AP (SPATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPATA5 Polyclonal antibody

SPATA5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32104-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AFG2 AAA ATPase homolog A, AFG2A, ATPase family gene 2 protein homolog A, EC:3.6.4.10, Ribosome biogenesis protein SPATA5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRT-4 cells, HEK-293T cells, U-251 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32104-1-AP targets SPATA5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36838

Product name: Recombinant human SPATA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 100-300 aa of BC048217

Sequence: WPMAGFPGGKVGLSEMAQKNVGVRPGDAIQVQPLVGAVLQAEEMDVALSDKDMEINEEELTGCILRKLDGKIVLPGNFLYCTFYGRPYKLQVLRVKGADGMILGGPQSDSDTDAQRMAFEQSSMETSSLELSLQLSQLDLEDTQIPTSRSTPYKPIDDRITNKASDVLLDVTQSPGDGSGLMLEEVTGLKCNFESAREGNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 5
别名 AFG2 AAA ATPase homolog A, AFG2A, ATPase family gene 2 protein homolog A, EC:3.6.4.10, Ribosome biogenesis protein SPATA5
计算分子量97, 86, 75 kDa
观测分子量100 kDa
GenBank蛋白编号BC048217
基因名称 SPATA5
Gene ID (NCBI) 166378
RRIDAB_3670193
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8NB90
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AAA+ ATPases SPATA5 is an ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability (PubMed:38554706). SPATA5 plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024, PubMed:38554706).

实验方案

Product Specific Protocols
IHC protocol for SPATA5 antibody 32104-1-APDownload protocol
WB protocol for SPATA5 antibody 32104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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