验证数据展示
经过测试的应用
| Positive WB detected in | RT-4 cells, HEK-293T cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32104-1-AP targets SPATA5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SPATA5 fusion protein Ag36838 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermatogenesis associated 5 |
| 别名 | SPAF, Ribosome biogenesis protein SPATA5, EC:3.6.4.10, ATPase family gene 2 protein homolog A, AFG2A |
| 计算分子量 | 97, 86, 75 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC048217 |
| 基因名称 | SPATA5 |
| Gene ID (NCBI) | 166378 |
| RRID | AB_3670193 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8NB90 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AAA+ ATPases SPATA5 is an ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability (PubMed:38554706). SPATA5 plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024, PubMed:38554706).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPATA5 antibody 32104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |