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验证数据展示

  • WB analysis of mouse testis using 16989-1-AP

    WB analysis of mouse testis using 16989-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPATA22 antibody included
  • IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    IHC staining of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16989-1-AP (SPATA22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPATA22 antibody included
  • IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    IP experiment of mouse testis using 16989-1-AP (same clone as 16989-1-PBS)

    IP result of anti-SPATA22 (IP:16989-1-AP, 3ug; Detection:16989-1-AP 1:500) with mouse testis tissue lysate 4800ug. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

    WB analysis using 16989-1-AP (same clone as 16989-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16989-1-AP (SPATA22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16989-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SPATA22 Polyclonal antibody, PBS Only

SPATA22 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16989-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPATA22 Ready-To-Use IHC Kit. SPATA22 primary antibody included.

产品信息

16989-1-PBS targets SPATA22 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10591

Product name: Recombinant human SPATA22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-363 aa of BC029483

Sequence: MKRSLNENSARSTAGCLPVPLFNQKKRNRQPLTSNPLKDDSGISTPSDNYDFPPLPTDWAWEAVNPELAPVMKTVDTGQIPHSVSRPLRSQDSVFNSIQSNTGRSQGGWSYRDGNKNTSLKTWNKNDFKPQCKRTNLVANDGKNSCPMSSGAQQQKQLRTPEPPNLSRNKETELLRQTHSSKISGCTMRGLDKNSALQTLKPNFQQNQYKKQMLDDIPEDNTLKETSLYQLQFKEKASSLRIISAVIESMKYWREHAQKTVLLFEVLAVLDSAVTPGPYYSKTFLMRDGKNTLPCVFYEIDRELPRLIRGRVHRCVGNYDQKKNIFQCVSVRPASVSEQKTFQAFVKIADVEMQYYINVMNET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 22
别名 NYD SP20, SPATA 22, Spermatogenesis-associated protein 22, Testis development protein NYD-SP20
计算分子量 363 aa, 41 kDa
观测分子量 44, 41 kDa
GenBank蛋白编号BC029483
基因名称 SPATA22
Gene ID (NCBI) 84690
RRIDAB_2286640
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NHS9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Spata22 (NYD-SP20), a novel vertebrate-specific gene, is required for meiotic progress in mouse germ cells. It is essential for one or more key events of early meiotic prophase, as homologous chromosomes of mutant germ cells do not achieve normal synapsis or repair meiotic DNA double-strand breaks.

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