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验证数据展示

  • WB analysis using 31808-1-AP

    WB analysis using 31808-1-AP

    Mouse testis tissue lysate was subjected to SDS PAGE followed by western blot with 31808-1-AP (SPATA18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 31808-1-AP

    IHC staining of rat testis using 31808-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31808-1-AP (SPATA18 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 31808-1-AP

    IHC staining of rat testis using 31808-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31808-1-AP (SPATA18 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SPATA18 Polyclonal antibody

SPATA18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31808-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

MIEAP, Mitochondria-eating protein, Spermatogenesis-associated protein 18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31808-1-AP targets SPATA18 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36227

Product name: Recombinant human SPATA18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-332 aa of BC025396

Sequence: LQDTFDRERHKDPSPRDRDMQQLDSNLNSTRSQCNQVQDDLVETEKNLEESKNRSAISLLAAEEEINQLKKQLKSLQAQEDARHRNTDQRSSENRRSEPWSLEERKREQWNSLKQNADQQDTEAMSDYKKQLRNLKEEIAVLSAEKSALQGRSSRSRSPSPAPRSRSCSRSRSASPSTAVKVRRPSPNRSKLSNVARKAALLSRFSDSYSQARLDAQCLLRRCIDKAETVQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 18 homolog (rat)
别名 MIEAP, Mitochondria-eating protein, Spermatogenesis-associated protein 18
计算分子量 538 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC025396
基因名称 SPATA18
Gene ID (NCBI) 132671
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TC71
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The modulation of mitophagy after DNA damage is independent of the type of DNA damage stimuli used and the protein spermatogenesis-associated protein 18 (SPATA18) is an important player in this process (PMID: 32453416; 21264221). SPATA18, also known as MIEAP, is a p53 transcriptional target and was previously reported to contribute to mitochondrial quality control by inducing intramitochondrial lysosome-like organelles after mitochondrial damage (PMID: 21264221).

实验方案

Product Specific Protocols
IHC protocol for SPATA18 antibody 31808-1-APDownload protocol
WB protocol for SPATA18 antibody 31808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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