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验证数据展示

  • IHC staining of human testis using 17357-1-AP

    IHC staining of human testis using 17357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 17357-1-AP

    IHC staining of human testis using 17357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 40x lens).

SPATA16 Polyclonal antibody

SPATA16 Polyclonal Antibody for IHC, ELISA
Cat No. 17357-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

NYD SP12, SPATA16, spermatogenesis associated 16

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17357-1-AP targets SPATA16 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10637

Product name: Recombinant human SPATA16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 221-570 aa of BC034496

Sequence: EDIASVASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQATVFRCLERYSEAARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKVKDAFTKTHPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTREDTVRQMETMGKRILPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPYLQDISQQEAELLQSLMADAMDTLEGRRNNNERVWNMIQKVGQIEDFLYQLEDSFLKTKKLRTARRQKTKMKRLQTVQQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 16
别名 NYD SP12, SPATA16, spermatogenesis associated 16
计算分子量 569 aa, 65 kDa
GenBank蛋白编号BC034496
基因名称 SPATA16
Gene ID (NCBI) 83893
RRIDAB_2878396
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXB7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPATA16, also named as NYD-SP12, belongs to the SPATA16 family. It is involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. SPATA16 localizes to Golgi apparatus and is primarily expressed in testis, with lower levels found in kidney and pancreas.

实验方案

Product Specific Protocols
IHC protocol for SPATA16 antibody 17357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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