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验证数据展示

  • WB analysis of COLO 320 using 21400-1-AP

    WB analysis of COLO 320 using 21400-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21400-1-AP

    IF Staining of HepG2 using 21400-1-AP

    Immunofluorescent analysis of HepG2 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human rectal cancer using 21400-1-AP

    IHC staining of human rectal cancer using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPATA13 antibody included
  • IHC staining of mouse small intestine using 21400-1-AP

    IHC staining of mouse small intestine using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPATA13 antibody included
  • IHC staining of rat small intestine using 21400-1-AP

    IHC staining of rat small intestine using 21400-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21400-1-AP (SPATA13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPATA13 antibody included

SPATA13 Polyclonal antibody

SPATA13 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21400-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Asef2, spermatogenesis associated 13, Spermatogenesis-associated protein 13

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, human placenta tissue
Positive IHC detected inhuman rectal cancer tissue, mouse small intestine tissue, mouse kidney tissue, rat kidney tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HEK-293 cells, Hela cells
Planning an IHC experiment? We recommend our IHCeasy SPATA13 Ready-To-Use IHC Kit. SPATA13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21400-1-AP targets SPATA13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16101

Product name: Recombinant human SPATA13 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC109291

Sequence: MTSASPEDQNAPVGCPKGARRRRPISVIGGVSLYGTNQTEELDNLLTQPASRPPMPAHQVPPYKAVSARFRPFTFSQSTPIGLDRVGRRRQMRASNVSSDGGTEPSALVDDNGSEEDFSYEDLCQASPRYLQPGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermatogenesis associated 13
别名 Asef2, spermatogenesis associated 13, Spermatogenesis-associated protein 13
计算分子量 652 aa, 75 kDa
观测分子量 75 kDa
GenBank蛋白编号BC109291
基因名称 SPATA13
Gene ID (NCBI) 221178
RRIDAB_10859783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96N96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SPATA13 antibody 21400-1-APDownload protocol
IHC protocol for SPATA13 antibody 21400-1-APDownload protocol
WB protocol for SPATA13 antibody 21400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Acta Biochim Biophys Sin (Shanghai)

Targeting the Spata13-TGFβRI interaction inhibits infection-driven capsular contracture via suppression of fibroblast activation.

Authors - Yiyin Tang
View Article
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