验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85906-4-RR targets SPARCL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SPARCL1 fusion protein Eg2837 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SPARC-like 1 (hevin) |
| 别名 | Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin) |
| 计算分子量 | 75 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | NM_004684.5 |
| 基因名称 | SPARCL1 |
| Gene ID (NCBI) | 8404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N4S1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPARCL1 antibody 85906-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |