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验证数据展示

  • WB analysis of human saliva using 67511-1-Ig

    WB analysis of human saliva using 67511-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67511-1-Ig (SPARCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SPARCL1 Monoclonal antibody

SPARCL1 Monoclonal Antibody for WB, ELISA
Cat No. 67511-1-Ig

产品说明书

CloneNo. 1A9E7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman saliva, Raji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67511-1-Ig targets SPARCL1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 SPARCL1 fusion protein Ag29092 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SPARC-like 1 (hevin)
别名 Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1
计算分子量 664 aa, 75 kDa
观测分子量 140 kDa
GenBank蛋白编号BC033721
基因名称 SPARCL1
Gene ID (NCBI) 8404
RRIDAB_2882732
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14515
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

实验方案

Product Specific Protocols
WB protocol for SPARCL1 antibody 67511-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols