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验证数据展示

  • IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPARC antibody included
  • IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPARC antibody included
  • WB analysis of A375 using 15274-1-AP (same clone as 15274-1-PBS)

    WB analysis of A375 using 15274-1-AP (same clone as 15274-1-PBS)

    WB result of SPARC antibody (15274-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPARC transfected A375 cells. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

  • WB analysis using 15274-1-AP (same clone as 15274-1-PBS)

    WB analysis using 15274-1-AP (same clone as 15274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15274-1-AP (SPARC antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

  • IP experiment of A375 using 15274-1-AP (same clone as 15274-1-PBS)

    IP experiment of A375 using 15274-1-AP (same clone as 15274-1-PBS)

    IP result of anti-SPARC (IP:15274-1-AP, 4ug; Detection:15274-1-AP 1:3000) with A375 cells lysate 1520 ug. This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    IHC staining of human stomach cancer using 15274-1-AP (same clone as 15274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15274-1-AP (SPARC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15274-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SPARC antibody included
  • Featured Product
  • KD/KO Validated

SPARC Polyclonal antibody, PBS Only

SPARC Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15274-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IP, Indirect ELISA

别名

Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, ON

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPARC Ready-To-Use IHC Kit. SPARC primary antibody included.

产品信息

15274-1-PBS targets SPARC in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag7390

Product name: Recombinant human SPARC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-303 aa of BC004974

Sequence: MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secreted protein, acidic, cysteine-rich (osteonectin)
别名 Basement membrane protein 40, Basement-membrane protein 40, BM 40, BM-40, ON
计算分子量 35 kDa
观测分子量35-43 kDa
GenBank蛋白编号BC004974
基因名称 SPARC
Gene ID (NCBI) 6678
RRIDAB_2194961
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09486
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Secreted Protein Acidic And Cysteine Rich (SPARC), also known as osteonectin or basement-membrane protein 40 (BM-40), is a secreted protein essential in the calcification of bone. 

What is the molecular weight of SPARC?

The calculated molecular mass of SPARC is 35 kDa. SPARC is a glycoprotein consisting of 303 amino acids in the cytoplasm and 286 amino acids after the signal sequence is cleaved in secretion. 

Where is SPARC expressed?

Osteoblasts secrete SPARC during bone formation, with high levels found in immature bone compared to mature bone that is in homeostasis (PMID: 2440898). SPARC is also secreted in adult mineralized tissues that have high turnover, such as osteoid and dentin, by cells other than osteoblasts. This includes bone marrow progenitor cells and hypertrophic chondrocytes but also endothelial cells and fibroblasts.

Though previously described as being exclusively expressed in mineralized tissue, it is now understood that SPARC is more widely expressed and is associated with high levels of collagen deposition, found in the extracellular matrix (ECM) of a number of tissues.

What is the function of SPARC?

SPARC contains both a collagen-binding domain and a hydroxyapatite (HA) binding region, so is thought to enhance mineralization by binding both collagen and HA crystals, causing the release of calcium ions (PMID: 26851678).

SPARC can bind to a number of different ECM proteins, particularly collagen, and may even influence the assembly of these proteins (PMID: 19798598). It is known to regulate interactions between cells and the ECM, meaning it plays a role in many important processes such as cell migration and proliferation.

What diseases are associated with SPARC?

Overexpression of SPARC has been identified in a number of different types of cancers (PMID: 18849185), although its role may vary. For example, in neuroblastomas SPARC acts as a suppressor but in gliomas, it causes greater invasion of the tumor. Mutations in SPARC can also lead to Osteogenesis Imperfecta, also known as brittle bone disease (PMID: 26027498).


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