验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, MCF-7 cells, SH-SY5Y cells, A-673 cells, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86527-1-RR targets SPAM1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag1682 Product name: Recombinant human SPAM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 212-511 aa of BC026163 Sequence: LLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SPAM1 |
| 别名 | EC:3.2.1.35, HYA1, Hyal PH20, HYAL1, HYAL3 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC026163 |
| 基因名称 | SPAM1 |
| Gene ID (NCBI) | 6677 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P38567 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.
