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SPAG11A Polyclonal antibody

SPAG11A Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16770-1-AP

Print datasheet

Synonyms

EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A

验证数据展示

  • WB analysis of human testis using 16770-1-AP

    WB analysis of human testis using 16770-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16770-1-AP

    IHC staining of human testis using 16770-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16770-1-AP

    IHC staining of human testis using 16770-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman testis tissue, human brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16770-1-AP targets SPAG11A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivitymouse
Immunogen SPAG11A fusion protein Ag9846 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 11A
Synonyms EP2, HE2, Protein EP2, SPAG11A, Sperm antigen HE2, sperm associated antigen 11A
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC058833
Gene Symbol SPAG11A
Gene ID (NCBI) 653423
RRIDAB_2286582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PDA7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sperm binding proteins and their C-terminal peptides of the Sperm AssociatedAntigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.

实验方案

Product Specific Protocols
WB protocol for SPAG11A antibody 16770-1-APDownload protocol
IHC protocol for SPAG11A antibody 16770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Reprod Biol Endocrinol

Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.

Authors - Pujianto Dwi A DA
View Article