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验证数据展示

  • IHC staining of human testis using 12829-1-AP

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12829-1-AP

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 40x lens).

SPACA1 Polyclonal antibody

SPACA1 Polyclonal Antibody for IHC, ELISA
Cat No. 12829-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA and More (1)

别名

SAMP32, SPACA1, sperm acrosome associated 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12829-1-AP targets SPACA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag3549

Product name: Recombinant human SPACA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 26-229 aa of BC029488

Sequence: SARGTNVTAAVQDAGLAHEGEGEEETENNDSETAENYAPPETEDVSNRNVVKEVEFGMCTVTCGIGVREVILTNGCPGGESKCVVRVEECRGPTDCGWGKPISESLESVRLACIHTSPLNRFKYMWKLLRQDQQSIILVNDSAILEVRKESHPLAFECDTLDNNEIVATIKFTVYTSSELQMRRSSLPATDAALIFVLTIGVII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm acrosome associated 1
别名 SAMP32, SPACA1, sperm acrosome associated 1
计算分子量 294 aa, 32 kDa
GenBank蛋白编号BC029488
基因名称 SPACA1
Gene ID (NCBI) 81833
RRIDAB_2877886
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPACA1, also named as SAMP32, is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. SPACA1 is regulated by phosphorylation.

实验方案

Product Specific Protocols
IHC protocol for SPACA1 antibody 12829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Free Radic Res

Heterozygous SOD1 deficiency in mice with an NZW background causes male infertility and an aberrant immune phenotype

Authors - Takujiro Homma
View Article