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验证数据展示

  • WB analysis of Jurkat using 25000-1-AP

    WB analysis of Jurkat using 25000-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 25000-1-AP

    WB analysis of K-562 using 25000-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25000-1-AP (SP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SP2 Polyclonal antibody

SP2 Polyclonal Antibody for WB, ELISA
Cat No. 25000-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA and More (1)

别名

KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, K-562 cells, C6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25000-1-AP targets SP2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用IHC
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag8060

Product name: Recombinant human SP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 303-606 aa of BC016680

Sequence: QQQVVQIPQQALRVVQAASATLPTVPQKPSQNFQIQAAEPTPTQVYIRTPSGEVQTVLVQDSPPATAAATSNTTCSSPASRAPHLSGTSKKHSAAILRKERPLPKIAPAGSIISLNAAQLAAAAQAMQTININGVQVQGVPVTITNTGGQQQLTVQNVSGNNLTISGLSPTQIQLQMEQALAGETQPGEKRRRMACTCPNCKDGEKRSGEQGKKKHVCHIPDCGKTFRKTSLLRAHVRLHTGERPFVCNWFFCGKRFTRSDELQRHARTHTGDKRFECAQCQKRFMRSDHLTKHYKTHLVTKNL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sp2 transcription factor
别名 KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2
计算分子量 65 kDa
观测分子量 72 kDa
GenBank蛋白编号BC016680
基因名称 SP2
Gene ID (NCBI) 6668
RRIDAB_2879839
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.

实验方案

Product Specific Protocols
WB protocol for SP2 antibody 25000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Commun Biol

ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer

Authors - Rebecca Garnham
View Article
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