验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HEK-293 cells, K-562 cells, C6 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
25000-1-AP targets SP2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, rat |
文献引用反应性 | human |
免疫原 | SP2 fusion protein Ag8060 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Sp2 transcription factor |
别名 | KIAA0048, SP2, Sp2 transcription factor, Transcription factor Sp2 |
计算分子量 | 65 kDa |
观测分子量 | 72 kDa |
GenBank蛋白编号 | BC016680 |
基因名称 | SP2 |
Gene ID (NCBI) | 6668 |
RRID | AB_2879839 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q02086 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SP2 also named as Sp2 transcription factor or KIAA0048 is a 613 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the sp1 C2H2-type zinc finger protein family. SP2 binds to box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites in nucleus . SP2 may regulate expression of the T-cell antigen receptor (TCR) gene.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SP2 antibody 25000-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |