验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, PC-3 cells, human brain tissue, Jurkat cells, K-562 cells, human testis tissue, HeLa cells, COLO 320 cells. |
| Positive IP detected in | HeLa cells |
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11502-1-AP targets SP110 in WB, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2042 Product name: Recombinant human SP110 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC019059 Sequence: MFTMTRAMEEALFQHFMHQKLGIAYAIHKPFPFFEGLLDNSIITKRMYMESLEACRNLIPVSRVVHNILTQLERTFNLSLLVTLFSQINLREYPNLVTIYRSFKRVGASYERQSRDTPILLEAPTGLAEGSSLHTPLALPPPQPPQPSCSPCAPRVSEPGTSSQQSDEILSESPSPSDPVLPLPALIQEGRSTSVTNDKLTSKMNAEEDSEEMPSLLTSTVQVASDNLIPQIRDKEDPQEMPHSPLGSMPEIRDNSPEPNDPEEPQEVSSTPSDKKGKKRKRCIWSTPKRRHKKKSLPRALSRHGIQKKLKRVDQVPQKKDDSTCNSTVETRAQKARTECARKSRSEEII 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SP110 nuclear body protein |
| 别名 | Speckled 110 kDa, SP110 nuclear body protein, IPR1, Interferon-induced protein 41/75, IFI75 |
| 计算分子量 | 689 aa, 79 kDa |
| 观测分子量 | 62-66 kDa |
| GenBank蛋白编号 | BC019059 |
| 基因名称 | SP110 |
| Gene ID (NCBI) | 3431 |
| RRID | AB_10863116 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HB58 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for SP110 antibody 11502-1-AP | Download protocol |
| WB protocol for SP110 antibody 11502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
