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验证数据展示

  • WB analysis using 31102-1-AP

    WB analysis using 31102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31102-1-AP (SP100 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SP100 Polyclonal antibody

SP100 Polyclonal Antibody for WB, ELISA
Cat No. 31102-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy SP100 Ready-To-Use IHC Kit. SP100 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31102-1-AP targets SP100 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag35193

Product name: Recombinant human SP100 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 141-300 aa of BC011562

Sequence: YKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCETEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELHNHGIQINSCSVRLVDIKKEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SP100 nuclear antigen
别名 DKFZp686E07254, FLJ00340, FLJ34579, Lysp100b, Nuclear autoantigen Sp 100, SP100, SP100 nuclear antigen, Speckled 100 kDa
计算分子量 879 aa, 100 kDa
观测分子量75-85 kDa
GenBank蛋白编号BC011562
基因名称 SP100
Gene ID (NCBI) 6672
RRIDAB_3669856
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP23497
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.

实验方案

Product Specific Protocols
WB protocol for SP100 antibody 31102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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