验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, COLO 320 cells, Caco-2 cells, Calu-1 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
27526-1-AP targets SOX9 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 |
CatNo: Ag26274 Product name: Recombinant human SOX9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 306-405 aa of NM_000346 Sequence: MVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 9 |
| 别名 | CMD1, CMPD1, SRA1, Transcription factor SOX 9, Transcription factor SOX-9 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 56 kDa, 68-75 kDa |
| GenBank蛋白编号 | NM_000346 |
| 基因名称 | SOX9 |
| Gene ID (NCBI) | 6662 |
| RRID | AB_3742237 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48436 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor. SOX9 can be ubiquitinated or SUMOylated to higher molecular weight (PMID: 24155239; 16307912; 16554309; 32070068)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SOX9 antibody 27526-1-AP | Download protocol |
| WB protocol for SOX9 antibody 27526-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Oleanolic acid cubic liquid crystal nanoparticle-based thermosensitive gel attenuates knee osteoarthritis symptoms in rats. | ||
Biochem Pharmacol SIS3 attenuates traumatic heterotopic ossification by targeting TGF-β2-driven endothelial-mesenchymal transition. |
