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验证数据展示

  • WB analysis using 86619-2-RR

    WB analysis using 86619-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86619-2-RR (SOX5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 86619-2-RR

    WB analysis of U-87 MG using 86619-2-RR

    WB result of SOX5 antibody (86619-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX5 transfected U-87 MG cells.

  • IF Staining of HepG2 using 86619-2-RR

    IF Staining of HepG2 using 86619-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOX5 antibody (86619-2-RR, Clone: 251512D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • KD/KO Validated

SOX5 Recombinant monoclonal antibody

SOX5 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86619-2-RR

产品说明书

CloneNo. 251512D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

L SOX5, Transcription factor SOX-5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, U-87 MG cells, NCI-H1299 cells
Positive IF/ICC detected inHepG2 cells, A549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86619-2-RR targets SOX5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4092

Product name: Recombinant human SOX5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-376 aa of BC029220

Sequence: DEVAQPLNLSAKPKTSDGKSPTSPTSPHMPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTKAIQEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKFSHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCLVDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLPSEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDYGSDSENHIAGQAN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SRY (sex determining region Y)-box 5
别名 L SOX5, Transcription factor SOX-5
计算分子量 763 aa, 84 kDa
观测分子量84-90 kDa
GenBank蛋白编号BC029220
基因名称 SOX5
Gene ID (NCBI) 6660
RRIDAB_3745013
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35711
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOX5 is a member of the SRY-related HMG-box (SOX) family of transcription factors, which play critical roles in cell fate determination and differentiation during embryonic development. SOX5 contains a highly conserved high-mobility group (HMG) DNA-binding domain that enables it to regulate target gene expression by bending DNA and facilitating chromatin remodeling. SOX5 is essential for chondrogenesis, neurogenesis, and gliogenesis, and its dysregulation has been implicated in various cancers and developmental disorders (PMID: 19647094, PMID: 30914279). SOX5 has two known protein isoforms: a short isoform of 48 kDa and a long isoform of 84 kDa (PMID: 21732189). This antibody detects the 84-90 kDa band and has passed KD/KO validation.

实验方案

Product Specific Protocols
IF protocol for SOX5 antibody 86619-2-RRDownload protocol
WB protocol for SOX5 antibody 86619-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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