SOX2 Recombinant antibody
SOX2 Recombinant Antibody for IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
230225G1
验证数据展示
经过测试的应用
Positive IF detected in | U-251 cells |
Planning an IHC experiment? We recommend our IHCeasy SOX2 Ready-To-Use IHC Kit. SOX2 primary antibody included.
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:150-1:600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83059-5-RR targets SOX2 in IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, ELISA |
Tested Reactivity | Human |
Immunogen | SOX2 fusion protein Ag0612 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SRY (sex determining region Y)-box 2 |
Synonyms | ANOP3, MCOPS3, SOX2, Transcription factor SOX 2 |
Calculated Molecular Weight | 34 kDa |
GenBank Accession Number | BC013923 |
Gene Symbol | SOX2 |
Gene ID (NCBI) | 6657 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P48431 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. Affinity purified rabbit anti-Sox2 antibody can be used to demonstrate pluripotency of ES and iPS cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human SOX2. a rare undiferentiated cell population that is intermingled with the Bergmann glia of the adult murine cerebellar cortex, expresses the stem cell markers Sox2 and Nestin, and lacks markers of glial or neuronal diferentiation. Sox2-expressing neural stem cells in the subgranular zone (SGZ) , a well-known stem cell niche of the adult brain.
实验方案
Product Specific Protocols | |
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IF protocol for SOX2 antibody 83059-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |