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SOX15 Polyclonal antibody

SOX15 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25415-1-AP

Print datasheet

Synonyms

Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27

验证数据展示

  • IHC staining of mouse embryo using 25415-1-AP

    IHC staining of mouse embryo using 25415-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 25415-1-AP (SOX15 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOX15 antibody included
  • IF Staining of mouse embryo using 25415-1-AP

    IF Staining of mouse embryo using 25415-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25415-1-AP (SOX15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue
Planning an IHC experiment? We recommend our IHCeasy SOX15 Ready-To-Use IHC Kit. SOX15 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25415-1-AP targets SOX15 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SOX15 fusion protein Ag22032 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 15
Synonyms Protein SOX 12, Protein SOX 15, Protein SOX 20, SOX12, SOX15, SOX20, SOX26, SOX27
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC000985
Gene Symbol SOX15
Gene ID (NCBI) 6665
RRIDAB_2880067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60248
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOX15, also named as SOX12, SOX20, is a 223 amino acid protein, which contains one HMG box DNA-binding domain. SOX15 is widely expressed in fetal and adult tissues and highest level is found in fetal spinal cord and adult brain and testis. SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling. Sox15 is required for skeletal muscle regeneration and is up regulated in the embryonic mouse testis.

实验方案

Product Specific Protocols
IHC protocol for SOX15 antibody 25415-1-APDownload protocol
IF protocol for SOX15 antibody 25415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Transcription factor SOX15 regulates stem cell pluripotency and promotes neural fate during differentiation by activating the neurogenic gene Hes5

Authors - Eun-Bee Choi
  • KO Validated
View Article