验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, mouse brain tissue, BxPC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23939-1-AP targets SOX12 in WB, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, ChIP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag21043 Product name: Recombinant human SOX12 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-315 aa of BC067361 Sequence: MVQQRGARAKRDGGPPPPGPGPAEEGAREPGWCKTPSGHIKRPMNAFMVWSQHERRKIMDQWPDMHNAEISKRLGRRWQLLQDSEKIPFVREAERLRLKHMADYPDYKYRPRKKSKGAPAKARPRPPGGSGGGSRLKPGPQLPGRGGRRAAGGPLGGGAAAPEDDDEDDDEELLEVRLVETPGRELWRMVPAGRAARGQAERAQGPSGEGAAAAAAASPTPSEDEEPEEEEEEAAAAEEGEEETVASGEESLGFLSRLPPGPAGLDCSALDRDPDLQPPSGTSHFEFPDYCTPEVTEMIAGDWRPSSIADLVFTY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 12 |
| 别名 | Protein SOX 22, Protein SOX-22, SOX22, Transcription factor SOX 12, Transcription factor SOX-12 |
| 计算分子量 | 315 aa, 34 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC067361 |
| 基因名称 | SOX12 |
| Gene ID (NCBI) | 6666 |
| RRID | AB_2879368 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O15370 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX12 antibody 23939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation | ||
Cell Death Dis The SOX12-YBX1-LDHA signaling axis drives metastasis in papillary thyroid carcinoma. | ||
Mol Ther Nucleic Acids lncRNA PRR34-AS1 promotes HCC development via modulating Wnt/β-catenin pathway by absorbing miR-296-5p and upregulating E2F2 and SOX12. | ||
Cancer Gene Ther LncRNA UCA1 promotes SOX12 expression in breast cancer by regulating m6A modification of miR-375 by METTL14 through DNA methylation. | ||
Cancer Biol Ther Hypoxia promotes non-small cell lung cancer cell stemness, migration, and invasion via promoting glycolysis by lactylation of SOX9 | ||
Mol Carcinog FOXN3 Downregulation in Colorectal Cancer Enhances Tumor Cell Stemness by Promoting EP300-Mediated Epigenetic Upregulation of SOX12 |
