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SOS1 Polyclonal antibody

SOS1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 55041-1-AP

Print datasheet

Synonyms

GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1

验证数据展示

  • WB analysis using 55041-1-AP

    WB analysis using 55041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 55041-1-AP

    IP experiment of mouse brain using 55041-1-AP

    IP result of anti-SOS1 (IP:55041-1-AP, 4ug; Detection:55041-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • WB analysis using 55041-1-AP

    WB analysis using 55041-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 55041-1-AP

    IHC staining of mouse colon using 55041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 55041-1-AP

    IHC staining of mouse colon using 55041-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 55041-1-AP

    IF Staining of HeLa using 55041-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOS1 antibody (55041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung cancer using 55041-1-AP

    IHC staining of human lung cancer using 55041-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

55041-1-AP targets SOS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name son of sevenless homolog 1 (Drosophila)
Synonyms GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1
Calculated Molecular Weight 152 kDa
Observed Molecular Weight152 kDa
GenBank Accession NumberNM_005633
Gene Symbol SOS1
Gene ID (NCBI) 6654
RRIDAB_10804659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07889
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.

实验方案

Product Specific Protocols
WB protocol for SOS1 antibody 55041-1-APDownload protocol
IHC protocol for SOS1 antibody 55041-1-APDownload protocol
IF protocol for SOS1 antibody 55041-1-APDownload protocol
IP protocol for SOS1 antibody 55041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
View Article
humanWB

Pathol Res Pract

KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway

Authors - Jishuang Ding
View Article
humanWB

Sci Adv

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis

Authors - Qiwei Jiang
View Article