验证数据展示
经过测试的应用
| Positive WB detected in | SK-N-SH cells, IMR-32 cells. SH-SY5Y cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32248-1-AP targets SORCS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37710 Product name: Recombinant human SORCS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-190 aa of NM_014978.2 Sequence: EITWDATGGPGRPAAPASRPPALSPLSPRAVASQWPEELASARRAAVLGRRAGPELLPQQGGGRGGEMQVEAGGTSPAGERRGRGIPAPAKLGGARRSRRAQPPITQERGDAWATAPADGSRGSRPLAKGSREEVKAPRAGGSAAEDLRLPSTSFAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sortilin-related VPS10 domain containing receptor 3 |
| 别名 | KIAA1059, VPS10 domain-containing receptor SorCS3 |
| 计算分子量 | 136kDa，1222aa |
| 观测分子量 | 160 kDa |
| GenBank蛋白编号 | NM_014978.2 |
| 基因名称 | SORCS3 |
| Gene ID (NCBI) | 22986 |
| RRID | AB_3742552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9UPU3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sortilin related VPS10 domain-containing receptor 3 (SorCS3) belongs to the vacuolar protein sorting 10 protein (VPS10p) receptor family. SorCS3 is a specific receptor for nerve growth factor (NGF) that regulates NGF signal transduction across membranes, and SorCS3 is mainly expressed in the hippocampus and cortex of the brain (PMID: 35393432).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SORCS3 antibody 32248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
