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SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 18839-1-AP

Print datasheet

Synonyms

hSorCS, SORCS1

验证数据展示

  • WB analysis of Y79 using 18839-1-AP

    WB analysis of Y79 using 18839-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 18839-1-AP

    FC experiment of HeLa using 18839-1-AP

    1X10^6 HeLa cells were stained with 0.2ug SORCS1 antibody (18839-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

经过测试的应用

Positive WB detected inY79 cells, HEK-293 cells, mouse brain tissue
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18839-1-AP targets SORCS1 in WB, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, WB,ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sortilin-related VPS10 domain containing receptor 1
Synonyms hSorCS, SORCS1
Calculated Molecular Weight 1168 aa, 130 kDa
Observed Molecular Weight 130 kDa, 60-80 kDa
GenBank Accession NumberBC131597
Gene Symbol SORCS1
Gene ID (NCBI) 114815
RRIDAB_2878564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WY21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.

实验方案

Product Specific Protocols
WB protocol for SORCS1 antibody 18839-1-APDownload protocol
FC protocol for SORCS1 antibody 18839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols