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  • Featured Product
  • KD/KO Validated

SORBS1 Polyclonal antibody

SORBS1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 13854-1-AP

Print datasheet

Synonyms

c Cbl associated protein, CAP, Ponsin, R85FL, SH3 domain protein 5, SH3D5, SH3P12, SORB1, SORBS1

验证数据展示

  • WB analysis of 3T3-L1 using 13854-1-AP

    WB analysis of 3T3-L1 using 13854-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13854-1-AP (SORBS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 13854-1-AP

    IP experiment of mouse liver using 13854-1-AP

    IP result of anti-SORBS1 (IP:13854-1-AP, 4ug; Detection:13854-1-AP 1:800) with mouse liver tissue lysate 4800ug.

经过测试的应用

Positive WB detected in3T3-L1 cells, HEK-293 cells, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13854-1-AP targets SORBS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, canine
Immunogen SORBS1 fusion protein Ag4828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorbin and SH3 domain containing 1
Synonyms c Cbl associated protein, CAP, Ponsin, R85FL, SH3 domain protein 5, SH3D5, SH3P12, SORB1, SORBS1
Calculated Molecular Weight 1292 aa, 142 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC042612
Gene Symbol SORBS1
Gene ID (NCBI) 10580
RRIDAB_11232224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BX66
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SORBS1 antibody 13854-1-APDownload protocol
IP protocol for SORBS1 antibody 13854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB，IHC

Front Cell Dev Biol

Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.

Authors - Yiyang Xu
  • KD Validated
View Article
mouseIF

Front Cell Neurosci

Integrative Cell Type-Specific Multi-Omics Approaches Reveal Impaired Programs of Glial Cell Differentiation in Mouse Culture Models of DM1.

Authors - Anchel González-Barriga
View Article
canineIF

PLoS One

Sorbin and SH3 domain-containing protein 2 (SORBS2) is a component of the acto-myosin ring at the apical junctional complex in epithelial cells.

Authors - Karin Fredriksson-Lidman
View Article
human,mouseWB

Sci Rep

STAP-2 facilitates insulin signaling through binding to CAP/c-Cbl and regulates adipocyte differentiation

Authors - Yuichi Sekine
View Article