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验证数据展示

  • IF Staining of HepG2 using 83519-2-RR (same clone as 83519-2-PBS)

    IF Staining of HepG2 using 83519-2-RR (same clone as 83519-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    IF Staining of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    FC experiment of HeLa using 83519-2-RR (same clone as 83519-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-2-RR, Clone:240462F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83519-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83519-2-RR

    Affinity and Kinetic Characterization of 83519-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83519-2-RR against Human SOD2 were performed. The affinity constant is 2.75 nM.

SOD2 Recombinant monoclonal antibody, PBS Only

SOD2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83519-2-PBS

产品说明书

CloneNo. 240462F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

SODM, 240462F8, EC:1.15.1.1, IPO B, IPOB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.

产品信息

83519-2-PBS targets SOD2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21388

Product name: Recombinant human SOD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-140 aa of BC016934

Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 superoxide dismutase 2, mitochondrial
别名 SODM, 240462F8, EC:1.15.1.1, IPO B, IPOB
计算分子量 25 kDa
GenBank蛋白编号BC016934
基因名称 SOD2
Gene ID (NCBI) 6648
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04179
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.

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