SOD2 Polyclonal antibody

SOD2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 24127-1-AP

Print datasheet

Synonyms

IPO B, Mn SOD, MNSOD, SOD2, SODM



经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24127-1-AP targets SOD2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC, CoIP, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, zebrafish, pig, Bovine
Immunogen SOD2 fusion protein Ag21388 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superoxide dismutase 2, mitochondrial
Synonyms IPO B, Mn SOD, MNSOD, SOD2, SODM
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
RRIDAB_2879437
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04179
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Superoxide dismutase 2 (SOD2), also known as MnSOD (manganese-dependent superoxide dismutase), is a 

mitochondrial enzyme that dismutates superoxide ions.


What is the molecular weight of SOD2? Is SOD2 post-translationally modified?

The molecular weight of SOD2 is 25 kDa. SOD2 acts as a homotetramer with manganese ion. SOD2 can be 

post-translationally modified, including phosphorylation, nitration, and acetylation. Phosphorylation of SOD2 

increases its enzymatic activity and occurs as part of the adaptive response to radiation stress (PMID: 23243068), 

while nitration and acetylation decrease SOD2 enzymatic activity (PMID: 20106845, 21172655).


What is the subcellular localization of superoxide dismutases?

SOD2 is the only superoxide dismutase that localizes to the mitochondrial matrix (PMID: 4578091). SOD1 is 

predominantly found in the cytoplasm and to a lesser extent in the mitochondrial intermembrane space and 

nucleus (PMID: 11507097), while SOD3 localizes extracellularly.


What is the role of SOD2 in neutralizing reactive oxygen species (ROS)?

Superoxide is a main form of ROS that originates directly from the electron transport chain in the mitochondrial 

matrix. SOD2 plays a crucial role in mitochondrial ROS homeostasis by catalyzing turnover of superoxide radicals 

to hydrogen peroxide and molecular oxygen. ROS are potent physiological signaling molecules but at high doses 

they contribute to the pathogenesis of many diseases, including cardiovascular and neurodegenerative diseases 

and cancer (PMID: 29669742). SOD2 also associates with mitochondrial DNA (mtDNA), protecting it from 

oxidative damage (PMID: 19228881).


What is the tissue expression pattern of SOD2?

SOD2 is ubiquitously expressed.


Can SOD2 be used as a marker of oxidative stress?

SOD2 is a key player in antioxidant defense and contributes to the regulation of oxidative stress. Defects in SOD2 

activity or levels can result in oxidative stress. However, SOD2 cannot be used as a marker of oxidative stress 

because its expression is not always altered by oxygen stress. 


实验方案

Product Specific Protocols
WB protocol for SOD2 antibody 24127-1-APDownload protocol
IHC protocol for SOD2 antibody 24127-1-APDownload protocol
IF protocol for SOD2 antibody 24127-1-APDownload protocol
IP protocol for SOD2 antibody 24127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

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Authors - Calvin S Carter
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Adv Sci (Weinh)

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Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
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miR-484 mediates oxidative stress-induced ovarian dysfunction and promotes granulosa cell apoptosis via SESN2 downregulation

Authors - Xiaofei Wang
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Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang