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- KD/KO Validated
SOD1 Polyclonal antibody
SOD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
215
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue |
Positive IP detected in | HEK-293 cells, HeLa cells |
Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells, HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
产品信息
10269-1-AP targets SOD1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | CoIP, ELISA, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, chicken, pig, canine, bovine |
Immunogen | SOD1 fusion protein Ag0335 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | superoxide dismutase 1, soluble |
Synonyms | ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn] |
Calculated molecular weight | 16 kDa |
Observed molecular weight | 16-20 kDa |
GenBank accession number | BC001034 |
Gene symbol | SOD1 |
Uniprot | P00441 |
Gene ID (NCBI) | 6647 |
RRID | AB_2193750 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for SOD1 antibody 10269-1-AP | Download protocol |
IHC protocol for SOD1 antibody 10269-1-AP | Download protocol |
IF protocol for SOD1 antibody 10269-1-AP | Download protocol |
IP protocol for SOD1 antibody 10269-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Nat Commun Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis. | ||
Microbiome Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets. | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. |