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- KD/KO Validated
SOD1 Polyclonal antibody
SOD1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
236
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HEK-293 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10269-1-AP targets SOD1 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB,IP,IHC,IF,ELISA,CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat, chicken, pig, canine, bovine |
| Immunogen | SOD1 fusion protein Ag0335 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | superoxide dismutase 1, soluble |
| Synonyms | ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn] |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC001034 |
| Gene Symbol | SOD1 |
| Gene ID (NCBI) | 6647 |
| RRID | AB_2193750 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00441 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IHC protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IF protocol for SOD1 antibody 10269-1-AP | Download protocol |
| IP protocol for SOD1 antibody 10269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons | ||
Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. | ||
Nat Commun Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis. | ||
Microbiome Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets. | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. |
