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  • Featured Product
  • KD/KO Validated

SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10269-1-AP

Print datasheet

Synonyms

ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]

验证数据展示

  • WB analysis of HEK-293 using 10269-1-AP

    WB analysis of HEK-293 using 10269-1-AP

    WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

  • WB analysis using 10269-1-AP

    WB analysis using 10269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10269-1-AP

    WB analysis using 10269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10269-1-AP

    WB analysis of HeLa using 10269-1-AP

    HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 10269-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IP experiment of HEK-293 using 10269-1-AP

    IP experiment of HEK-293 using 10269-1-AP

    IP Result of anti-SOD1 (IP:10269-1-AP, 3ug; Detection:10269-1-AP 1:1500) with HEK-293 cells lysate 1000ug.

  • IP experiment of HeLa using 10269-1-AP

    IP experiment of HeLa using 10269-1-AP

    HeLa lysates prepared and IP of SOD1 performed using 2.0 μg of 10269-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IHC staining of human liver cancer using 10269-1-AP

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOD1 antibody included
  • IHC staining of human liver cancer using 10269-1-AP

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SOD1 antibody included
  • IF Staining of human kidney using 10269-1-AP

    IF Staining of human kidney using 10269-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 10269-1-AP

    IF Staining of HeLa using 10269-1-AP

    HeLa WT cells (yellow outline) and SOD1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 10269-1-AP. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IF Staining of HEK-293 using 10269-1-AP

    IF Staining of HEK-293 using 10269-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10269-1-AP (SOD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HEK-293 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue
Positive IP detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy SOD1 Ready-To-Use IHC Kit. SOD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

10269-1-AP targets SOD1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, ELISA, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, pig, canine, bovine
Immunogen SOD1 fusion protein Ag0335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superoxide dismutase 1, soluble
Synonyms ALS1, homodimer, IPOA, SOD, SOD1, Superoxide dismutase [Cu Zn]
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC001034
Gene symbol SOD1
UniprotP00441
Gene ID (NCBI) 6647
RRIDAB_2193750
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

实验方案

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-APDownload protocol
IHC protocol for SOD1 antibody 10269-1-APDownload protocol
IF protocol for SOD1 antibody 10269-1-APDownload protocol
IP protocol for SOD1 antibody 10269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

Authors - Zaida Álvarez
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mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
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mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
humanWB

Nat Commun

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.

Authors - Yun Liao
View Article
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
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mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang
View Article