验证数据展示
经过测试的应用
| Positive WB detected in | A-204 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16038-1-AP targets SOCS2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag8909 Product name: Recombinant human SOCS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC010399 Sequence: MTLRCLEPSGNGGEGTRSQWGTAGSAEEPSPQAARLAKALRELGQTGWYWGSMTVNEAKEKLKEAPEGTFLIRDSSHSDYLLTISVKTSAGPTNLRIEYQDGKFRLDSIICVKSKLKQFDSVVHLIDYYVQMCKDKRTGPEAPRNGTVHLYLTKPLYTSAPSLQHLCRLTINKCTGAIWGLPLPTRLKDYLEEYKFQV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | suppressor of cytokine signaling 2 |
| 别名 | CIS2, CIS-2, Cytokine-inducible SH2 protein 2, SOCS 2, SOCS-2 |
| 计算分子量 | 198 aa, 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC010399 |
| 基因名称 | SOCS2 |
| Gene ID (NCBI) | 8835 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14508 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOCS2 is the substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as EPOR and GHR (PMID: 11781573; 21980433; 25505247; 31182716; 34857742).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOCS2 antibody 16038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
