×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 29369-1-AP (same clone as 29369-1-PBS)

    WB analysis using 29369-1-AP (same clone as 29369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29369-1-AP (SOAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29369-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 29369-1-AP (same clone as 29369-1-PBS)

    IHC staining of human placenta using 29369-1-AP (same clone as 29369-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29369-1-AP (SOAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29369-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29369-1-AP (same clone as 29369-1-PBS)

    IF Staining of HeLa using 29369-1-AP (same clone as 29369-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT1 antibody (29369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29369-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29369-1-AP (same clone as 29369-1-PBS)

    IF Staining of HepG2 using 29369-1-AP (same clone as 29369-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOAT1/ACAT1 antibody (29369-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29369-1-PBS in a different storage buffer formulation.

SOAT1/ACAT1 Polyclonal antibody, PBS Only

SOAT1/ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29369-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SOAT1, ACACT, ACAT, ACAT-1, Acyl-coenzyme A:cholesterol acyltransferase 1

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

29369-1-PBS targets SOAT1/ACAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30945

Product name: Recombinant human SOAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-140 aa of BC028940

Sequence: MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterol O-acyltransferase 1
别名 SOAT1, ACACT, ACAT, ACAT-1, Acyl-coenzyme A:cholesterol acyltransferase 1
计算分子量 550 aa, 65 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC028940
基因名称 SOAT1/ACAT1
Gene ID (NCBI) 6646
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35610
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOAT1 (Sterol O-acyltransferase 1), also known as ACAT1 (Acyl-Coenzyme A: cholesterol acyltransferase 1), is a crucial endoplasmic reticulum-resident enzyme that plays a central role in cellular cholesterol homeostasis. Its primary function is to catalyze the esterification of free cholesterol with long-chain fatty acids, forming cholesteryl esters. This process serves two key purposes: it prevents the toxic buildup of free cholesterol in cellular membranes and creates neutral lipid droplets for cholesterol storage. SOAT1 is ubiquitously expressed, with high activity in the steroidogenic tissues, and macrophages. Due to its role in facilitating cholesterol ester accumulation in macrophages and its overexpression in various cancers, SOAT1 has emerged as a promising therapeutic target for the treatment of atherosclerosis, neurodegenerative diseases, and cancer.

||
New chat

Able™

正在加载，请稍候...