验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, SW480 cells, mouse liver tiisue, rat liver tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29369-1-AP targets SOAT1/ACAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag30945 Product name: Recombinant human SOAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC028940 Sequence: MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterol O-acyltransferase 1 |
| 别名 | SOAT1, ACACT, ACAT, ACAT-1, Acyl-coenzyme A:cholesterol acyltransferase 1 |
| 计算分子量 | 550 aa, 65 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC028940 |
| 基因名称 | SOAT1/ACAT1 |
| Gene ID (NCBI) | 6646 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35610 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOAT1 (Sterol O-acyltransferase 1), also known as ACAT1 (Acyl-Coenzyme A: cholesterol acyltransferase 1), is a crucial endoplasmic reticulum-resident enzyme that plays a central role in cellular cholesterol homeostasis. Its primary function is to catalyze the esterification of free cholesterol with long-chain fatty acids, forming cholesteryl esters. This process serves two key purposes: it prevents the toxic buildup of free cholesterol in cellular membranes and creates neutral lipid droplets for cholesterol storage. SOAT1 is ubiquitously expressed, with high activity in the steroidogenic tissues, and macrophages. Due to its role in facilitating cholesterol ester accumulation in macrophages and its overexpression in various cancers, SOAT1 has emerged as a promising therapeutic target for the treatment of atherosclerosis, neurodegenerative diseases, and cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SOAT1/ACAT1 antibody 29369-1-AP | Download protocol |
| IHC protocol for SOAT1/ACAT1 antibody 29369-1-AP | Download protocol |
| WB protocol for SOAT1/ACAT1 antibody 29369-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
