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SOAT1/ACAT1 Polyclonal antibody

SOAT1/ACAT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 13202-1-AP

Print datasheet

Synonyms

ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1

验证数据展示

  • WB analysis using 13202-1-AP

    WB analysis using 13202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13202-1-AP (SOAT1/ACAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HuH-7 cells, SW480 cells, THP-1 cells, mouse liver tissue, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13202-1-AP targets SOAT1/ACAT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SOAT1/ACAT1 fusion protein Ag3944 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterol O-acyltransferase 1
Synonyms ACACT, ACACT1, ACAT, ACAT 1, ACAT1, Cholesterol acyltransferase 1, RP11 215I23.2, SOAT, SOAT1, STAT, sterol O acyltransferase 1
Calculated Molecular Weight 550 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC028940
Gene Symbol SOAT1
Gene ID (NCBI) 6646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sterol O-acyltransferase 1 (SOAT1/ACAT1) catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol (PubMed:16154994,16647063, 32433614, 32944968). It plays a role in lipoprotein assembly and dietary cholesterol absorption (PubMed:16154994, 9020103). SOAT1 has three isoforms with molecular weights of 65, 58, and 57 kDa.

实验方案

Product Specific Protocols
WB protocol for SOAT1/ACAT1 antibody 13202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols