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验证数据展示

  • WB analysis using 11494-1-AP

    WB analysis using 11494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11494-1-AP (SNX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 11494-1-AP

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 11494-1-AP

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX8 Polyclonal antibody

SNX8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Mvp1, SNX8, sorting nexin 8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11494-1-AP targets SNX8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2055

Product name: Recombinant human SNX8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 166-465 aa of BC021565

Sequence: SEDVVLKLFLSFSGSDVQNKLKESAQCVGDEFLNCKLATRAKDFLPADIQAQFAISRELIRNIYNSFHKLRDRAERIASRAIDNAADLLIFGKELSAIGSDTTPLPSWAALNSSTWGSLKQALKGLSVEFALLADKAAQQGKQEENDVVEKLNLFLDLLQSYKDLCERHEKGVLHKHQRALHKYSLMKRQMMSATAQNREPESVEQLESRIVEQENAIQTMELRNYFSLYCLHQETQLIHVYLPLTSHILRAFVNSQIQGHKEMSKVWNDLRPKLSCLFAGPHSTLTPPCSPPEDGLCPH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 8
别名 Mvp1, SNX8, sorting nexin 8
计算分子量 465 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC021565
基因名称 SNX8
Gene ID (NCBI) 29886
RRIDAB_2923557
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5X2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexin 8 (SNX8) is a member of the sorting nexin family. Members of the sorting nexin family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX8 plays a role in the endosome-to-Golgi transport (PMID: 19782049). SNX8 can be used as a potential target for treating lysosomal storage disorder (PMID: 38519472).

实验方案

Product Specific Protocols
IHC protocol for SNX8 antibody 11494-1-APDownload protocol
WB protocol for SNX8 antibody 11494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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