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  • Featured Product
  • KD/KO Validated

SNX32 Polyclonal antibody

SNX32 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 25763-1-AP

Print datasheet

Synonyms

SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B

验证数据展示

  • WB analysis of HeLa using 25763-1-AP

    WB analysis of HeLa using 25763-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25763-1-AP (SNX32 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25763-1-AP

    IHC staining of mouse brain using 25763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25763-1-AP

    IHC staining of mouse brain using 25763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25763-1-AP (SNX32 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25763-1-AP targets SNX32 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, pig
Immunogen SNX32 fusion protein Ag22653 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 32
Synonyms SNX32, SNX6B, sorting nexin 32, Sorting nexin 6B
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC040981
Gene Symbol SNX32
Gene ID (NCBI) 254122
RRIDAB_2880229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86XE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX32 (Sorting nexin-32, also known as SNX6B) may be involved in several stages of intracellular trafficking.

实验方案

Product Specific Protocols
WB protocol for SNX32 antibody 25763-1-APDownload protocol
IHC protocol for SNX32 antibody 25763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,pigCoIP,WB,IF,IP

J Virol

SNX32 is a host restriction factor that degrades African swine fever virus CP204L via the RAB1B-dependent autophagy pathway

Authors - Wenping Yang
  • KD Validated
View Article