验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A549 cells, HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10772-1-AP targets SNX3 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SNX3 fusion protein Ag1160 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 3 |
| 别名 | Sorting nexin-3, sorting nexin 3, SDP3, Protein SDP3, MCOPS8 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | BC008444 |
| 基因名称 | SNX3 |
| Gene ID (NCBI) | 8724 |
| RRID | AB_2192691 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60493 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNX3 is a member of the sorting nexin family of proteins regulating membrane traffic. SNX3 regulates endosomal function through its PX (phox homology)-domain-mediated interaction with PtdIns(3)P (PMID: 11433298). It is a potential regulator of neurite formation and may play an important role in the development and function of the central nervous system (PMID: 19576982; 20817026).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX3 antibody 10772-1-AP | Download protocol |
| IHC protocol for SNX3 antibody 10772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation.
| ||
Acta Pharm Sin B Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis | ||
Nat Commun SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.
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Environ Int HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity | ||
Cell Death Differ Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.
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Oncogene EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers.
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