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验证数据展示

  • WB analysis of COLO 320 using 13180-1-AP

    WB analysis of COLO 320 using 13180-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13180-1-AP (SNX20 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 13180-1-AP

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 13180-1-AP

    IHC staining of human placenta using 13180-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13180-1-AP (SNX20 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of COLO 320 using 13180-1-AP

    IP experiment of COLO 320 using 13180-1-AP

    IP result of anti-SNX20 (IP:13180-1-AP, 4ug; Detection:13180-1-AP 1:300) with COLO 320 cells lysate 2000ug.

SNX20 Polyclonal antibody

SNX20 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13180-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

SLIC 1, SLIC1, SNX20, sorting nexin 20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, mouse thymus tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman placenta tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13180-1-AP targets SNX20 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNX20 fusion protein Ag3847 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 20
别名 SLIC 1, SLIC1, SNX20, sorting nexin 20
计算分子量 316 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC027944
基因名称 SNX20
Gene ID (NCBI) 124460
RRIDAB_10638911
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z614
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNX20 antibody 13180-1-APDownload protocol
IHC protocol for SNX20 antibody 13180-1-APDownload protocol
IP protocol for SNX20 antibody 13180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols