验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11737-1-AP targets SNX2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2259 Product name: Recombinant human SNX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 171-520 aa of BC003382 Sequence: SMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHVSVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKREAEAKMMVANKPDKIQQAKNEIREWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKTVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 2 |
| 别名 | Sorting nexin-2, Transformation-related gene 9 protein, TRG9, TRG-9 |
| 计算分子量 | 519 aa, 58 kDa |
| 观测分子量 | ~70 kda |
| GenBank蛋白编号 | BC003382 |
| 基因名称 | SNX2 |
| Gene ID (NCBI) | 6643 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sorting nexin 2 (SNX2) belongs to the sorting nexin family, characterized by the presence of a phox (PX) domain that binds phosphoinositides and mediates intracellular trafficking. It regulates the cell-surface expression and degradation of epidermal growth factor receptor (EGFR) through endosomal sorting (PMID: 14978220). SNX2 also participates in retrograde transport from endosomes to the trans-Golgi network and maintains cellular homeostasis (PMID: 38033809).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX2 antibody 11737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
