验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, human placenta tissue, L02 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
21946-1-AP targets SNX18 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SNX18 fusion protein Ag16645 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sorting nexin 18 |
| 别名 | SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18 |
| 计算分子量 | 628 aa, 69 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC060791 |
| 基因名称 | SNX18 |
| Gene ID (NCBI) | 112574 |
| RRID | AB_11182387 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RF0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNX18 antibody 21946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
