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验证数据展示

  • WB analysis of MCF-7 using 21946-1-AP

    WB analysis of MCF-7 using 21946-1-AP

    WB result of SNX18 antibody (21946-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX18 transfected MCF-7 cells.

  • WB analysis using 21946-1-AP

    WB analysis using 21946-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 21946-1-AP

    IHC staining of human placenta using 21946-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX18 antibody included
  • IHC staining of human placenta using 21946-1-AP

    IHC staining of human placenta using 21946-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX18 antibody included
  • IHC staining of mouse bladder using 21946-1-AP

    IHC staining of mouse bladder using 21946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX18 antibody included
  • IHC staining of mouse kidney using 21946-1-AP

    IHC staining of mouse kidney using 21946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNX18 antibody included
  • Featured Product
  • KD/KO Validated

SNX18 Polyclonal antibody

SNX18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21946-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

SH3 and PX domain-containing protein 3B, SH3PXD3B, Sorting nexin-18

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-231 cells
Positive IHC detected inhuman placenta tissue, mouse kidney tissue, mouse bladder tissue, rat bladder tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SNX18 Ready-To-Use IHC Kit. SNX18 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

21946-1-AP targets SNX18 in WB, IHC, ELISA, IB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag16645

Product name: Recombinant human SNX18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 55-284 aa of BC060791

Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 18
别名 SH3 and PX domain-containing protein 3B, SH3PXD3B, Sorting nexin-18
计算分子量 628 aa, 69 kDa
观测分子量70 kDa
GenBank蛋白编号BC060791
基因名称 SNX18
Gene ID (NCBI) 112574
RRIDAB_11182387
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

实验方案

Product Specific Protocols
IHC protocol for SNX18 antibody 21946-1-APDownload protocol
WB protocol for SNX18 antibody 21946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIB

Adv Sci (Weinh)

Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53.

Authors - Roberta Cacciatore
View Article
humanWB

Cell Chem Biol

A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway.

Authors - Bide Tong
View Article
humanWB

Commun Biol

SNX9 family mediates βarrestin-independent GPCR endocytosis

Authors - Valeria L Robleto
View Article
humanWB

bioRxiv

Interactome mapping in human excitatory neurons reveals novel risk genes and pathways in Alzheimer's disease.

Authors - Xiaomu Wei
View Article
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