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验证数据展示

  • WB analysis using 16049-1-AP

    WB analysis using 16049-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16049-1-AP

    WB analysis of HeLa using 16049-1-AP

    WB result of SNX15 antibody (16049-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX15 transfected HeLa cells.

  • WB analysis using 16049-1-AP

    WB analysis using 16049-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SNX15 Polyclonal antibody

SNX15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16049-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HSAF001435, sorting nexin 15, Sorting nexin-15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, human testis tissue, SH-SY5Y cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16049-1-AP targets SNX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9006

Product name: Recombinant human SNX15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-342 aa of BC009897

Sequence: MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKKDPEDVKEVVVWKRYSDFRKLHGDLAYTHRNLFRRLEEFPAFPRAQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLKEFFRGGEVTRPLEVSRDLHILPPPLIPTPPPDDPRLSQLLPAERRGLEELEVPVDPPPSSPAQEALDLLFNCESTEEASGSPARGPLTEAELALFDPFSKEEGAAPSPTHVAELATMEVESARLDQEPWEPGGQEEEEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVHVLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 15
别名 HSAF001435, sorting nexin 15, Sorting nexin-15
计算分子量 342 aa, 38 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC009897
基因名称 SNX15
Gene ID (NCBI) 29907
RRIDAB_2192367
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

实验方案

Product Specific Protocols
WB protocol for SNX15 antibody 16049-1-APDownload protocol
IHC protocol for SNX15 antibody 16049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols