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验证数据展示

  • WB analysis using 16049-1-AP

    WB analysis using 16049-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16049-1-AP

    WB analysis of HeLa using 16049-1-AP

    WB result of SNX15 antibody (16049-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX15 transfected HeLa cells.

  • WB analysis using 16049-1-AP

    WB analysis using 16049-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16049-1-AP (SNX15 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 16049-1-AP

    IHC staining of mouse kidney using 16049-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16049-1-AP (SNX15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

SNX15 Polyclonal antibody

SNX15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16049-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HSAF001435, sorting nexin 15, Sorting nexin-15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, human testis tissue, SH-SY5Y cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16049-1-AP targets SNX15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNX15 fusion protein Ag9006 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 15
别名 HSAF001435, sorting nexin 15, Sorting nexin-15
计算分子量 342 aa, 38 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC009897
基因名称 SNX15
Gene ID (NCBI) 29907
RRIDAB_2192367
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

实验方案

Product Specific Protocols
WB protocol for SNX15 antibody 16049-1-APDownload protocol
IHC protocol for SNX15 antibody 16049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols