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验证数据展示

  • WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)

    WB analysis using 67280-1-Ig (same clone as 67280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    IHC staining of human colon cancer using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67280-1-Ig (SNRPN/SNRPB antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51682-1

    Cytometric bead array sample test of MP51682-1

    Cytometric bead array sample test of MP51682-1, SNRPN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67280-1-PBS. Detection antibody: 67280-2-PBS.

  • IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)

    IF Staining of SH-SY5Y using 67280-1-Ig (same clone as 67280-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SNRPN/SNRPB antibody (67280-1-Ig, Clone: 1H2F4 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 67280-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51682-1

    Cytometric bead array standard curve of MP51682-1

    Cytometric bead array standard curve of MP51682-1, SNRPN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67280-1-PBS. Detection antibody: 67280-2-PBS. Standard:Ag14030. Range: 0.781-100 ng/mL.

SNRPN/SNRPB Monoclonal antibody, PBS Only (Capture)

SNRPN/SNRPB Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67280-1-PBS

产品说明书

CloneNo. 1H2F4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

SNRPN, 1H2F4, HCERN3, SM D, Small nuclear ribonucleoprotein-associated protein N

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67280-1-PBS targets SNRPN/SNRPB as part of a matched antibody pair:

MP51682-1: 67280-1-PBS capture and 67280-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14030

Product name: Recombinant human SNRPN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-240 aa of BC000611

Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATASIAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPPGMRPPPPGIRGPPPPGMRPPRP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 small nuclear ribonucleoprotein polypeptide N
别名 SNRPN, 1H2F4, HCERN3, SM D, Small nuclear ribonucleoprotein-associated protein N
计算分子量 240 aa, 25 kDa
观测分子量 27-29 kDa
GenBank蛋白编号BC000611
基因名称 SNRPN
Gene ID (NCBI) 6638
RRIDAB_2882548
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP63162
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SNRPN, or SNURF-SNRPN, is a bicistronic imprinted gene that encodes 2 polypeptides, the SmN splicing factor, which is involved in RNA processing, and the SNRPN upstream reading frame (SNURF) polypeptide.The SNRPN gene is transcribed exclusively from the paternally inherited chromosome and shows highest expression in brain and heart. SNRPN may play a role in etiology of Prader-Willi syndrome. This antibody recognizes SNRPN and SNRPB due to high amino acid sequence homology.

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