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SNRPE Polyclonal antibody

SNRPE Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20407-1-AP

Print datasheet

Synonyms

Sm E, Sm protein E, SME, snRNP E, SNRPE

验证数据展示

  • WB analysis using 20407-1-AP

    WB analysis using 20407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20407-1-AP (SNRPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 20407-1-AP

    IHC staining of mouse brain using 20407-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20407-1-AP (SNRPE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse placenta tissue, human placenta tissue, human brain tissue
Positive IHC detected inmouse brain tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20407-1-AP targets SNRPE in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SNRPE fusion protein Ag14227 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide E
Synonyms Sm E, Sm protein E, SME, snRNP E, SNRPE
Calculated Molecular Weight 92 aa, 11 kDa
Observed Molecular Weight 11 kDa
GenBank Accession NumberBC002639
Gene Symbol SNRPE
Gene ID (NCBI) 6635
RRIDAB_10699882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The snRNP E (SNRPE)protein is one of several proteins associated with the U family of small nuclear RNAs that are involved in RNA processing. It functions in the U7 snRNP complex that is involved in histone 3'-end processing and also associated with snRNP U1, U2, U4/U6 and U5. This antibody raises against the full length of human SNRPE, and can recognize two bands of SNRPE, include a 9 kDa band(isoform) and a 11kDa band(SNRPE)

实验方案

Product Specific Protocols
WB protocol for SNRPE antibody 20407-1-APDownload protocol
IHC protocol for SNRPE antibody 20407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Am J Hum Genet

Mutations in SNRPE, which encodes a core protein of the spliceosome, cause autosomal-dominant hypotrichosis simplex.

Authors - Sandra M Pasternack
View Article
humanWB,IHC

J Pathol

A novel protein-based prognostic signature improves risk stratification to guide clinical management in early-stage lung adenocarcinoma patients.

Authors - Elena Martínez-Terroba
View Article